Novel Fluorogenic substrates containing Bimane system for microdetermination of angiotensin I converting enzyme.

Abstract

As a sensitive fluormetric assay for the activity of angiotensin converting enzyme, bimane-peptides containing tryptophan, i.e., 1,7-dioxo-2,5,6-trimethyl-1 H,7H-pyrazolo[1,2-.alpha.]pyrazol-3-yl-methylthiomethylcarbonyl-glycyl (or L-phenylalany)-L-tryptophyl-L-leucine (or L-proline), were synthesized and shown to be potent fluorogenic substrates for the micro-determination of angiotensin I converting enzyme activity.