Participation of a DNA-RNA hybrid complex in in vivo genetic transcription.
- 1 March 1966
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 55 (3), 635-641
- https://doi.org/10.1073/pnas.55.3.635
Abstract
A complex of the replicative form of [phi] X-174 DNA (RF-DNA) and messenger RNA was detected in [phi] X-174 infected Escherichla coli. This complex has characteristics similar to those which were found in in vitro systems where the replicative form of [phi]X-174 DNA was used as a template for an E. coli DNA-depen-dent RNA polymerase. The RF-DNA and the RNA are combined in an RNase-resistant structure which survives deproteinization by phenoichloroform and Duponol. The RNase-resistant structure involves the coding strand of RF-DNA. Heating or formamide treatment of the complex separates the RF-DNA and the RNA. It is concluded that the DNA-RNA hybrid complex is involved in the process of genetic transcription.This publication has 15 references indexed in Scilit:
- A DNA-RNA complex as an intermediate of in vitro genetic transcription.Proceedings of the National Academy of Sciences, 1965
- THE ENZYMATIC SYNTHESIS OF A CIRCULAR DNA-RNA HYBRIDProceedings of the National Academy of Sciences, 1964
- ON „NATURAL” DNA-RNA COMPLEXES IN PHAGE-INFECTED E. COLIProceedings of the National Academy of Sciences, 1964
- A Deoxyribonucleic Acid-associated Ribonucleic Acid from Drosophila melanogasterJournal of Biological Chemistry, 1964
- Mechanism of RNA polymerase action: Formation of DNA-RNA hybrids with single-stranded templatesJournal of Molecular Biology, 1964
- In Vitro synthesis and properties of a φX DNA-RNA hybridJournal of Molecular Biology, 1964
- DNA CIRCULARITY AND THE MECHANISM OF STRAND SELECTION IN THE GENERATION OF GENETIC MESSAGESProceedings of the National Academy of Sciences, 1964
- RESTRICTION OF IN VIVO GENETIC TRANSCRIPTION TO ONE OF THE COMPLEMENTARY STRANDS OF DNAProceedings of the National Academy of Sciences, 1963
- A NATURALLY OCCURRING DNA-RNA COMPLEX FROM NEUROSPORA CRASSAProceedings of the National Academy of Sciences, 1962
- THE OCCURRENCE OF NATURAL DNA-RNA COMPLEXES IN E. COLI INFECTED WITH T2Proceedings of the National Academy of Sciences, 1961