The effect of staphylococcal leucocidin on the leucocyte

Abstract

The S component and the F component of leucocidin are both adsorbed by the leucocyte from solutions containing either or both components. Treatment of the leucocyte with leucocidin does not decrease the amount of nucleic acid sedimented by high-speed centrifuging of homogenized leucocytes. The lipids of the leucocyte that are extractable with chloroform-methanol have been analysed and the phospholipids partially separated on silicic acid. The composition and chromatographic behavior of the lipids is not changed by pretreatment of the cells with leucocidin. Treatment with leucocidin decreases the rate of glycolysis in leucocytes and macrophages but leucocidin has no action on glycolysis in homogenates.