Action of 2-deoxy-D-glucose on frog gastric mucosa

Abstract

2-Deoxyglucose (2-DG) has been found to inhibit chloride and acid secretion by the in vitro frog mucosa, with a fall in short-circuit current and potential difference and a rise in resistance. The ATP levels and phosphohexoisomerase activity were essentially unchanged following 2-DG treatment. 3-Methyl-O-glucose uptake was reduced by about 50% following preincubation with 2-DG. The O₂ consumption was only slightly reduced with 10 mmoles 2-DG, but the CO₂ ratio from glucose-6-C¹⁴/glucose-1-C¹⁴ fell from 0.98 to 0.37, indicating activation of the hexosemonophosphate (HMP) shunt.