Gallocyanin-Chrome Alum Counterstaining of Golgi-Kopsch Impregnations
- 1 January 1983
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 58 (3), 171-175
- https://doi.org/10.3109/10520298309066779
Abstract
A simple technique is described for counterstaining Golgi-Kopsch impregnations. The sections are first stabilized by the method of Geisert and Updyke and then stained in 0.15% gallocyanin and 5% chromium potassium sulfate for 45 minutes at 55-60 C. The sections are then rinsed, dehydrated to 70% ethanol, cleared in terpineol, mounted and coverslipped. This procedure results in a light to medium blue stain of those cells not impregnated by the silver chromate. The major advantages of this procedure over earlier methods are: (1) the counterstain does not fade and (2) since no differentiation is required, many sections may be stained simultaneously.This publication has 13 references indexed in Scilit:
- Exposure to lines of only one orientation modifies dendritic morphology of cells in the visual cortex of the catJournal of Comparative Neurology, 1982
- The Golgi MethodsPublished by Springer Nature ,1981
- Chemical Stabilization of Golgi Silver Chromate ImpregnationsStain Technology, 1977
- Staining for Autoradiography of the Central Nervous SystemStain Technology, 1972
- Thermoelectric Freezing for Attaching Nitrocellulose Blocks to the Microtome StageStain Technology, 1965
- A Self-Limiting, Highly Selective Chromium-Hematoxylin StainStain Technology, 1965
- Basic Dye Counterstaining of Sections Impregnated by the Golgi-Cox MethodStain Technology, 1964
- Loss of Silver Grains from Radioautographs Stained by Gallocyanin-Chrome AlumStain Technology, 1962
- Une combinaison de deux vieilles méthodes histologiques pour le système nerveux centralEuropean Neurology, 1956
- Alcoholic Thionin Counterstaining Following Golgi Dichromate-Silver ImpregnationStain Technology, 1954