LASER FLOW CYTOMETRIC STUDIES ON THE INTRACELLULAR FLUORESCENCE OF ANTHRACYCLINES
- 1 January 1980
- journal article
- research article
- Vol. 40 (11), 3895-3900
Abstract
A laser flow cytometer was used to excite and quantitate the intracellular fluorescence of cells [mouse leukemia P-388, human neoplastic T cell CCRF-CEM, human neoplastic B cell LAZ-OO7] exposed in vitro and in vivo to various anthracyclines [used as antineoplastic drugs]. In cells exposed to adriamycin (ADR), intracellular drug fluorescence appeared slowly and reached a peak after 4 h of incubation. Cells incubated with 10 .mu.g/ml were 5 times more fluorescent than were cells incubated with 1 .mu.g/ml. Cells exposed to daunomycin were 2-4 times more fluorescent than were cells similarly exposed to ADR: the intracellular appearance of daunomycin fluorescence was much more rapid. Cells exposed to N-trifluoroacetyladriamycin and carminomycin had higher amounts of intracellular fluorescence (2-4 times); peak values were reached much more rapidly than in cells exposed to ADR. In cells exposed to rubidazone, fluorescence increased 2- to 4-fold with increased drug concentration and length of exposure. Nogalamycin fluorescence reached a peak after 60 min of incubation; a 10-fold increase in drug concentration increased fluorescence only 2-fold. In animals given injections of ADR (4 mg/kg) and sacrificed after 3 h, drug fluorescence could be detected in tumor and spleen cells. Fluorescence in heart nuclei was barely recognizable. Incubation of isolated nuclei in ADR (1 .mu.g/ml) showed that bone marrow and heart nuclei had greater amounts of ADR fluorescence (2- to 3-fold) than did spleen or liver nuclei similarly treated. The use of laser flow cytometry for monitoring intracellular anthracycline transport, binding and efflux is demonstrated.This publication has 5 references indexed in Scilit:
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