The Chemistry of Anti-Pernicious Anæmia Factors: Part I: The Liberation of Phosphorus as Phosthate From Vitamin B12 by Acid Hydrolysis
- 1 September 1949
- journal article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 1 (1), 287-291
- https://doi.org/10.1111/j.2042-7158.1949.tb12416.x
Abstract
Phosphate is found in acid hydrolysates of vitamin B12. Such hydrolysates are deeply coloured and, in order to determine phosphate in them colorimetrically, it has been necessary to develop a new application of the unidimensional paper-strip partition chromatogram technique. The atomic ratio of cobalt: phosphorus in vitamin B12 is found to be 1:1. From a comparison of this result (3 above) with the values recorded by Smith for the anti-pernicious anæmia factor of Smith and Parker6, it is concluded that the latter compound is not identical with vitamin B12.Keywords
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