A new combination of protecting groups and links for encoded synthetic libraries suited for consecutive tests on the solid phase and in solution
- 1 February 1996
- journal article
- Published by Springer Nature in Molecular Diversity
- Vol. 1 (2), 109-112
- https://doi.org/10.1007/bf01721325
Abstract
A strategy for high-throughput evaluation of combinatorial compound libraries is reported, which circumvents the necessity to test complex mixtures. The method is based on a new combination of protecting groups, solid-phase linker and tags. The bulk of the library first undergoes a binding assay with the components grafted on beads. A selection of beads carrying strong ligands is stripped from the labelled target and distributed into microvessels. The ligands are cleaved and rinsed into microeluates. Subsequently, a more detailed characterization with a functional assay in solution determines the best performers, which are identified through the peptidic tag left behind on the corresponding mother bead.Keywords
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