Reconstitution of genetically regulated responses against random and ordered synthetic polypeptides by methylated bovine serum albumin as analyzed by isoelectric focusing
- 1 September 1976
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 6 (9), 618-623
- https://doi.org/10.1002/eji.1830060905
Abstract
In previous publications it was shown by avidity measurements, cross-reactivity patterns and genetic analyses, that the tetrapeptide T-T-G-G is the immunodominant epitope of the synthetic polypeptide (T, G)-A–L. In the present study this close immunological relationship between the random multichain copolymer (T, G)-A–L and the ordered analogue (T-T-G-G)-A–L is extended by two additional criteria. First, the immune response against (T-T-G-G)-A–L in H-2k nonresponder mouse strains can be reconstituted to high antibody levels by complexing this antigen to methylated bovine serum albumin, as was tested earlier for (T, G)-A–L. The antibodies elicited upon reconstitution in both antigenic systems are directed mainly against the same determinant, T-T-G-G. Second, isoelectric focusing analysis of specific antisera developed with radiolabeled antigen revealed restricted 7 S IgG antibody populations in high responder and reconstituted high and low responder mice. The spectra were found to be of similar complexity in the (T, G)-A–L and in the (T-T-G-G)-A–L system. From these data it was concluded that the repertoires of specific B cells to T-T-G-G are very similar in high and low responder strains, and the defect in the H-2k low responder systems should be located at the level of T-B cell cooperation.This publication has 32 references indexed in Scilit:
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