Histochemical Studies of Leukocytes from an Inflammatory Exudate. Glycogen and Phosphorylase

Abstract

The glycogen content and the phosphorylase activity of migrated leukocytes in a "skin window" were studied by using the periodic acid - Schiff technique and the phosphorylase reaction of TAKEUCHL A sequence was observed from large neutrophils containing little glycogen to intensely stained neutrophils, morphologically resembling those in the blood. By a budding process the latter cell type was seen to form free globular cytoplasmic fragments. An increasing glycogen content was also observed in the macrophages. Phosphorylase activity was demonstrated in both neutrophils and macrophages. In the neutrophils the activity increased with the progress of the inflammatory reaction.