The Combined Effect of 3-Methylcholanthrene and N,N′-2,7-fluorenylenebisacetamide on the Induction of Cancer of the Glandular Stomach of the Rat

Abstract

This experiment tested in the same rat the combined effect of two procedures for the induction of cancer of the glandular stomach: 1) the intramural injection of 3-methylcholanthrene (3-MCA) into the antrum and fundus and 2) the oral administration of N,N′-2,7-fluorenylenebisacetamide (2,7-FAA) mixed with the diet. This combination induced an increased number of gastric sarcomas over the 3-MCA injection method alone. The combined procedure also induced more carcinomas of the glandular stomach than the administration of either 2,7-FAA or 3-MCA alone, but the additional carcinomas were frequently mixed with sarcomas. As expected, tumors and precancerous lesions, as well as atrophy and inflammatory lesions of many other organs and tissues, occurred in animals that ingested the 2,7-FAA; of particular note were sarcomas of the abdominal wall in relation to the cotton sutures, an adenomatous neoplastic lesion of the prostate gland, and carcinomas of the ethmoturbinals, nasolacrimal duct, canal of uterine cervix, and urinary bladder.