Organization of kappa light chain genes in germ-line and somatic tissue.
- 1 February 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (2), 1106-1110
- https://doi.org/10.1073/pnas.77.2.1106
Abstract
The organization of the mouse L chain genes in germ-line (sperm) and somatic (embryo) tissues was studied. A plasmid containing a DNA insert coding for the MOPC 167 myeloma K chain was constructed and the Southern blotting technique was used to determine the organization of K variable [V] and constant (C) region genes. In the haploid genome of the mouse, there was only 1 C region gene detectable and it had the same organization in sperm and embryo DNA. There were several V region genes in sperm and embryo that were related to the Vk167 gene. The organization of the V genes in sperm and embryo DNA was identical. There is apparently no rearrangement of V region genes (minigenes) during early embryogenesis.Keywords
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