Somatic embryogenesis and plant regeneration from leaf tissues of Panicum maximum Jacq.
- 1 January 1981
- journal article
- research article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 59 (5), 275-280
- https://doi.org/10.1007/bf00264979
Abstract
Somatic embryogenesis was induced in proliferating leaf segments of Panicum maximum Jacq., cultured on Murashige and Skoog's nutrient medium containing 2,4-dichlorophenoxyacetic acid and coconut milk. The embryoids gave rise to plants on a medium containing gibberellic acid. The plants were successfully transplanted to soil and grown to maturity. Histological examination of proliferating leaves showed that the embryogenic callus tissue was formed by divisions in cells of the lower epidermis as well as the mesophyll tissue. The regenerated plants showed the normal chromosome number of 2n=4x=32.Keywords
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