Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae.
- 1 December 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (24), 8839-8843
- https://doi.org/10.1073/pnas.84.24.8839
Abstract
A yeast nuclear extract supported transcription from the CYC1 and PYK1 promoter. Transcription was initiated in vitro at or near sites used in vivo. Deletion of "TATA" sequences abolished the reaction. .alpha.-Amanitin (10 .mu.g/ml) and chloride (100 mM) were highly inhibitory.This publication has 20 references indexed in Scilit:
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