Membrane potential and resistance changes induced in salivary gland acinar cells by microiontophoretic application of acetylcholine and adrenergic agonists

Abstract

The effects of microiontophoretic applications of catecholamines and acetylcholine on parotid acinar cell membrane potential and resistance were investigated using intracellular microelectrode recording in superfused segments of mouse parotid or rat submandibular glands. Short pulses of acetylcholine and α-adrenergic agonists had similar effects, consisting of a marked decrease in membrane resistance accompanied by an initial depolarization or hyperpolarization depending on the level of the resting membrane potential. This initial response was followed by a slow hyperpolarization occurring at a time when the resistance was increasing towards the prestimulation level. The equilibrium potential for the initial potential change caused by excitation of the cholinergic receptors was investigated directly by setting the membrane potential at different levels by injecting direct current and stimulating the same cell repeatedly with equal doses of acetylcholine. The equilibrium potential was found to be about −55 mV. The delayed hyperpolarization could not be reversed by passing hyperpolarizing current, but actually increased in size with higher membrane potentials. The minimum latency of the effect of acetylcholine or α-adrenergic agonists was 200–500 msec. Excitation of β-adrenoceptors caused, after a long latency of several seconds, a small depolarization. Epinephrine induced a combined α- and β-adrenergic response, with the α-component predominating. Blocking the α-adrenoceptors with phentolamine revealed the β-adrenergic depolarization, while blocking the β-adrenoceptors with propranolol caused the components of the α-adrenergic response to become more pronounced. All three receptors (α- and β-adrenoceptors and cholinergic receptors) were present in individual acini.