Transcription control of the aroP gene in Escherichia coli K-12: analysis of operator mutants
Open Access
- 31 December 1986
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 169 (1), 386-393
- https://doi.org/10.1128/jb.169.1.386-393.1987
Abstract
The nucleotide sequence of the region containing the promoter-operator for the aroP gene was determined. The start site of aroP transcription was identified by using S1 nuclease mapping and primer extension techniques. Examination of the nucleotide sequence revealed the presence of two "TYR R" boxes which are similar to those identified in the regulatory regions of other genes in the tyrR regulon. Bisulfite-induced aroP operator-constitutive mutants were analyzed, and the base-pair changes responsible for alterations in aroP regulation were located within these boxes.This publication has 47 references indexed in Scilit:
- Nucleotide sequence and transcription of the phenylalanine and tyrosine operons of Escherichia coli K12Journal of Molecular Biology, 1984
- Role of DNA regions flanking the tryptophan promoter of Escherichia coli II. Insertion of lac operator fragmentsGene, 1984
- Plasmid vectors for the selection of promotersGene, 1984
- A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragmentsGene, 1982
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980
- Extraction of nucleic acids from agarose gelsAnalytical Biochemistry, 1980
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and MuJournal of Molecular Biology, 1976
- Uptake of amino acids by Salmonella typhimuriumArchives of Biochemistry and Biophysics, 1964
- Sur la biosynthese de la β-galactosidase (lactase) chez Escherichia coli. La specificite de l'inductionBiochimica et Biophysica Acta, 1951