Regulation of beta-galactoside phosphate accumulation in Streptococcus pyogenes by an expulsion mechanism.
- 1 September 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (9), 5497-5501
- https://doi.org/10.1073/pnas.77.9.5497
Abstract
S. pyogenes pregrown on lactose took up glucose, lactose, or methyl .beta.-D-thiogalactopyranoside (MeSGal or TMG) by a phosphoenolpyruvate-dependent phosphotransferase system. MeSGal accumulated in the cell as MeSGal-phosphate (MeSGalP). Three effects were noted when various sugars were added to MeSGal preloaded cells: no decrease in intracellular MeSGalP concentration after addition of fructose, sucrose, o-nitrophenyl-.beta.-D-galactoside, glycerol, 6-deoxyglucose, .alpha.-methyl D-glucoside, 2-deoxygalactose, glucose I-phosphate, or glucose 6-phosphate; slow loss of preaccumulated MeSGalP evoked by lactose, 2-deoxy-D-glucose, or unlabeled MeSGal; and a short lag followed by extremely rapid expulsion of intracellular MeSGalP elicited by glucose or mannose and a slower expulsion elicited by glucosamine. The expelled compound was free MeSGal, indicating the involvement of dephosphorylation in the expulsion mechanism. Deoxyglucose inhibited the expulsion evoked by mannose, and prepoisoning of cells with fluoride or arsenate prevented the glucose-dependent expulsion. The expulsion is due to activation of an expulsion mechanism rather than to turnover of MeSGalP and leak of internal MeSGal with concomitant inhibiiton of MeSGal influx. The results suggest the need for phosphotransferase-dependent translocation of a preferential sugar or accumulation of the sugar catabolite for expulsion activation. The significance of the expulsion mechanism in synthesis regulation of enzymes involved in carbohydrate utilization is proposed.This publication has 26 references indexed in Scilit:
- Regulation of carbohydrate uptake in gram-positive bacteria.Journal of Biological Chemistry, 1976
- Effect of Sugar Analogues on Growth, Sugar Utilization, and Acid Production by Streptococcus mutansJournal of Dental Research, 1975
- Cyclic guanosine 3':5'-monophosphate in Escherichia coli and Bacillus lichenformis.1974
- Phosphoenolpyruvate-Dependent Glucose Transport in Oral StreptococciJournal of Dental Research, 1973
- NaF inhibition of phosphorylation and dephosphorylation involved in α-methyl-D glucoside transport in E. coli K 12. A pH dependant phenomenon sensitive to uncoupling agentsBiochimie, 1972
- Energization of Active Transport by Escherichia coliJournal of Biological Chemistry, 1972
- Local and non-local interactions of fluxes mediated by the glucose and galactoside permeases of Escherichia coliBiochimica et Biophysica Acta (BBA) - Biomembranes, 1971
- Inhibition of β-galactoside transport by substrates of the glucose transport system in Escherichia coliBiochimica et Biophysica Acta (BBA) - Biomembranes, 1967
- SUGAR PHOSPHATE PHOSPHOHYDROLASE .I. SUBSTRATE SPECIFICITY INTRACELLULAR LOCALIZATION AND PURIFICATION FROM NEISSERIA MENINGITIDIS1967
- Carbohydrate transport in Staphylococcus aureusBiochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis, 1966