Clinical applications of luminescent assays for enzymes and enzyme labels
- 1 January 1989
- journal article
- review article
- Published by Wiley in Journal of Clinical Laboratory Analysis
- Vol. 3 (5), 316-322
- https://doi.org/10.1002/jcla.1860030511
Abstract
A wide range of luminescent assays for enzyme labels has been developed. The clinical application of luminescent assays for horseradish peroxidase, alkaline phosphatase, xanthine oxidase, beta‐D galactosidase, and glucose 6‐phosphate dehydrogenase is discussed. The enzyme luminescent methods provide improved sensitivity compared with colorimetric and fluorimetric assays. A new reagent, dioxetane phosphate, has provided a simple and extremely sensitive assay for alkaline phosphatase. The available instruments and new photographic detection techniques for luminescent assays are briefly discussed.Keywords
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