Introduction of Specific Cleavages into RNAs of RNA Bacteriophages for Determination of Base Sequences
- 1 March 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (3), 742-745
- https://doi.org/10.1073/pnas.71.3.742
Abstract
Large fragments of R17, MS2, and Qbeta RNAs were obtained reproducibly by limited digestion by ribonuclease T1. After digestion of R17 and MS2 RNAs, two pairs of large fragments were obtained that probably resulted from specific cleavages of the whole molecules. The cleavages of MS2 RNA were produced at points 36 and 47% from the 5' end.Keywords
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