Quantitative Studies of the Mutagenesis of Toxoplasma gondii
- 1 June 1979
- journal article
- research article
- Published by JSTOR in Journal of Parasitology
- Vol. 65 (3), 364-370
- https://doi.org/10.2307/3280274
Abstract
The induction of mutants resistant to 5-fluorodeoxyuridine (FUDR) was used to measure the efficiency of various physical and chemical mutagens on extracellular and intracellular T. gondii. The frequency of resistant mutant was measured by plaque assay in human fibroblast cultures in the presence and absence of FUDR. When considered as a function of lethality, the most efficient mutagenesis was obtained with nitrosoguanidine treatment of extracellular parasites and with ethylmethane sulfonate treatment of actively growing intracellular parasites. Each of these treatments increased the frequency of FUDR-resistant mutants from < 1 to > 200/million parasites. UV irradiation, X-rays and the alkylating mustard ICR-191 also induced FUDR-resistant mutants in a dose-dependent fashion.This publication has 10 references indexed in Scilit:
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