A New Technic for the Production of an in Vivo Labeled Fibrinogen
Open Access
- 1 April 1967
- journal article
- research article
- Published by American Society of Hematology in Blood
- Vol. 29 (4), 517-525
- https://doi.org/10.1182/blood.v29.4.517.517
Abstract
The fact that in vitro labeled proteins, as a rule, exhibit faster turnover rates than in vivo labeled materials led us to explore means of obtaining in vivo labeled fibrinogen of high specific activity. It was found that defibrination of the rat provides a stimulus for the liver to regenerate fibrinogen at an accelerated rate. Administration of seleno75 methionine shortly after thrombin-induced defibrination of the animal resulted in the incorporation of large quantities of the label. The rate of incorporation was further increased if the amino acid was administered as a slow infusion during the entire period of fibrinogen regeneration. In addition, prior nephrectomy of the animal would appear to result in a slight increase in specific activity of the fibrinogen preparation obtained. The results of these studies indicate that defibrination, nephrectomy, and the prolonged infusion of the labeled amino acid selenomethionine provided us with a technic for obtaining a biosynthetically labeled, γ-emitting, fibrinogen preparation of high specific activity.This publication has 9 references indexed in Scilit:
- Cellular Site for Fibrinogen SynthesisJAMA, 1964
- IN VIVO BEHAVIOR OF I131-FIBRINOGENJCI Insight, 1963
- ALBUMINE ALS VORSTUFE VON GLOBULINEN IM NORMALEN ORGANISMUS DER RATTE1955
- SYNTHESIS OF ALL PLASMA PROTEIN FRACTIONS EXCEPT GAMMA GLOBULINS BY THE LIVERThe Journal of Experimental Medicine, 1954
- ZONE PAPER ELECTROPHORESIS STUDIES ON RADIO-IODINATED HUMAN SERUM ALBUMIN 1JCI Insight, 1954
- TRACER EXPERIMENTS WITH I131 LABELED HUMAN SERUM ALBUMIN: DISTRIBUTION AND DEGRADATION STUDIESJCI Insight, 1953
- STUDIES IN METHIONINE METABOLISM. III. THE FATE OF INTRAVENOUSLY ADMINISTERED S35-LABELED-METHIONINE IN NORMAL ADULT MALES, IN PATIENTS WITH CHRONIC HEPATIC DISEASE, “IDIOPATHIC” HYPOPROTEINEMIA AND CUSHING'S SYNDROME 1JCI Insight, 1950
- METHIONINE LABELED WITH RADIOACTIVE SULFUR AS AN INDICATOR OF PROTEIN FORMATION IN THE HEPATECTOMIZED DOGJournal of Biological Chemistry, 1947
- PLASMA PROTEINS: THEIR SOURCE, PRODUCTION AND UTILIZATIONPhysiological Reviews, 1940