Cleavage of single-stranded DNA by the A and A*proteins of bacteriophage φX174
Open Access
- 20 December 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 7 (8), 2177-2188
- https://doi.org/10.1093/nar/7.8.2177
Abstract
The purified A protein and A* protein of bacteriophage φX174 have been tested for endonucleaae activity on single stranded viral φX174 DNA. The A protein (55.000 daltons) nicks single-stranded DNA in the same way and at the same place as it does superhelical RFI DNA, at the origin of DNA replication. The A* protein (37.000 daltons) can cleave the single-stranded viral DNA at many different sites. It has however a strong preference for the origin of replication. Both proteins generate 3′OH ends and blocked 5′ termini at the nick site.Keywords
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