AN INDOLEACETIC ACID 'OXIDASE' SYSTEM IN THE MYCELIUM OF MELAMPSORA LINI (PERS.) LEV.

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Abstract
Flax rust mycelium was grown on cotyledons of flax in a modified Knop's medium. The mycelium produced and apparently secreted a typical peroxidase-mediated indoleacetic acid 'oxidase'. Decarboxylation of radioactive indoleacetate (—C14OOH) was stimulated by Mn++ (10−4 and 10−3M), dichlorophenol (10−4M), and resorcinol (10−4M). Catechol (10−4M) and pyrogallol (10−4M) inhibited the reaction. Moreover, pyrogallol competitively inhibited the reaction in the presence of resorcinol. All four phenols increased oxygen uptake by the mycelium, catechol and pyrogallol being the most effective. Cu++ (10−3M) inhibited the reaction by 60%; cyanide (10−3M) and diethydithiocarbamate (2 × 10−4M) were almost completely inhibitory. The results are discussed briefly in relation to host–parasite interactions and the control of indoleacetic acid oxidase activity in vivo.

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