Stoichiometric Relation between Liver-Receptor, Intrinsic Factor and Vitamin B12.

Abstract

“Receptors”for intrinsic factor exist in rat, bull, and rabbit liver homogenate precipitate, and remain intact after lyophilization and acetone-washing of the homogenate. There appears to be a stoichiometric relation between receptors, intrinsic factor, and Vit. B₁₂. Within stated limits, an incubation system of receptors, intrinsic factor, and/or Co⁶⁰-B₁₂ may prove useful for assay of intrinsic factor, of the quantity of free B₁₂ in biological fluids, and of receptors.