SURFACE EFFECTS ON BLOOD-CLOTTING COMPONENTS AS DETERMINED BY ZETA-POTENTIALS. I1

Abstract

The electrokinetic effects of blood, blood components and certain anticoagulants in contact with silica and silicone-coated capillaries were investigated using the streaming potential technique to obtain the zeta potentials. The apparatus, theory and technique have been described by Jones and Wood (Jour. Chem. Phys. 13(3): 106-121. 1945). The zeta potentials for protein solns. in vitreous silica and silicone were practically identical at pH 6.9. A comparison of the zeta potentials of plasma with those of plasma fractions indicated that either or both beta-globulin or fibrinogen were the surface-coating proteins in plasma. Heparin caused the zeta-potentials of whole blood, serum, plasma, gamma-globulins, beta-globulins, fibrinogen, thrombin, and thromboplastin to become more negative but had no effect on bovine crystalline albumin. Two other anticoagulants, paritol and phosphatide inhibitor, made the zeta-potential of fibrinogen more negati.ve in a manner similar to heparin, whereas 1,5-pentanediol, which inhibits the actions of thrombin on fibrinogen, probably had no effect on the zeta-potential of blood.