Fluorescence analysis of cells using a laser light source
- 1 March 1979
- journal article
- Published by Springer Nature in Cell Biochemistry and Biophysics
- Vol. 1 (1), 1-13
- https://doi.org/10.1007/bf02785052
Abstract
A quantitative microfluorometric instrument is described that employs a helium cadmium laser (442 nm) as the illumination source. The instrument consists of a double grating monochromator in front of a gallium arsenide photomultiplier that is interfaced with a desktop computer. The versatility of the instrument in making quantitative nucleic acid measurements on acridine orange and Feulgen-Schiff stained cells is demonstrated. The ploidy levels of several populations are easily determined, and the Feulgen fluorescent emissions are considerably greater than those obtained with a standard mercury lamp.Keywords
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