The deoxyribonucleic acid of Micrococcus radiodurans
- 1 December 1966
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 101 (3), 647-650
- https://doi.org/10.1042/bj1010647
Abstract
The DNA of Micrococcus radiodurans was prepared by three methods. Although the recovery of DNA varied considerably, the percentage molar base ratios of the DNA from the three preparations were essentially the same: guanine, 33+/-2; adenine, 18+/-1; cytosine, 33+/-2; thymine, 17+/-1. Base compositions calculated from T(m) values and from density in caesium chloride gradients also yielded guanine+cytosine contents of 66 and 68% of total bases respectively. No unusual bases were observed. The S(20,w) values were characteristic of high-molecular-weight DNA. Electron microscopy showed the purified DNA in long strands; occasionally these were coiled.Keywords
This publication has 10 references indexed in Scilit:
- Uracil in formic acid hydrolysates of deoxyribonucleic acidBiochemical Journal, 1965
- Similarity of Repair of Ionizing and Ultra-Violet Radiation Damage in Micrococcus radioduransNature, 1965
- A Comparison of the Initial Actions of Spleen Deoxyribonuclease and Pancreatic DeoxyribonucleaseJournal of Biological Chemistry, 1965
- Radiation Resistance and Deoxyribonucleic Acid Base Composition of Micrococcus radioduransNature, 1964
- The resistance of Micrococcus radiodurans to ultraviolet radiationBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1964
- [Preparation and length measurements of the total desoxyribonucleic acid content of T2 bacteriophages].1962
- Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperatureJournal of Molecular Biology, 1962
- Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsClJournal of Molecular Biology, 1962
- A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acidBiochemical Journal, 1956
- The estimation of phosphorusBiochemical Journal, 1940