Regulation of 25 Hydroxyvitamin D31-Hydroxylase in Serum-Free Monolayer Culture of Mouse Kidney
- 1 March 1982
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 110 (3), 1073-1075
- https://doi.org/10.1210/endo-110-3-1073
Abstract
Primary monolayer cultures of cells from normal mouse kidney were initiated and maintained in serum-free medium supplemented with insulin, transferrin and prostaglandin E1. Renal epithelial cells grow to confluence without detectable growth of fibroblasts. These cells contain an active 25(OH)D 3 1-hydroxylase with half-maximal formation of 1,25(OH>2D3 achieved at a substrate concentration of 13.3 nM. Activity of this enzyme is increased by low calcium medium or parathyroid hormone and decreased by high calcium medium, high phosphate medium or 1,25(HO)2D3Keywords
This publication has 11 references indexed in Scilit:
- INSULIN PERMITS PARATHYROID HORMONE STIMULATION OF 1,25-DIHYDROXYVITAMIN D3PRODUCTION IN CULTURED KIDNEYCELLSEndocrinology, 1981
- Measurement of mammalian 25-hydroxyvitamin D3 24R-and 1 alpha-hydroxylase.Proceedings of the National Academy of Sciences, 1981
- Transporting Renal Epithelium: Culture in Hormonally Defined Serum-Free MediumScience, 1980
- In vitro metabolism of 25-hydroxyvitamin D 3 by isolated rat kidney cellsProceedings of the National Academy of Sciences, 1980
- Kinetic behavior of 25-hydroxyvitamin D-1-hydroxylase and -24-hydroxylase in rat kidney mitochondria.Journal of Biological Chemistry, 1979
- Regulation of the metabolism of 25-hydroxyvitamin D3 in primary cultures of chick kidney cells.JCI Insight, 1979
- Regulation of the hydroxylation of 25-hydroxyvitamin D3 in vivo and in primary cultures of chick kidney cells.Journal of Biological Chemistry, 1979
- The isolation and characterization of the vitamin D-binding protein from rat serum.Journal of Biological Chemistry, 1978
- The Regulation of 24,25-Dihydroxyvitamin D3 Production in Cultures of Monkey Kidney Cells11Endocrinology, 1977
- Isolation of an inhibitor of 25-hydroxyvitamin D3-1-hydroxylase from rat serumBiochemistry, 1976