Apparent half-lives of sugar transport proteins inSaccharomyces cerevisiae
- 1 March 1978
- journal article
- research article
- Published by Springer Nature in Folia Microbiologica
- Vol. 23 (2), 118-125
- https://doi.org/10.1007/bf02915311
Abstract
Using incubation in the presence of 0.4mm cycloheximide the half-lives of the principal membrane transport proteins in baker's yeast were found to be: more than 24 h for the constitutive glucose carrier, 2.2 h for the inducible galactose carrier, 1.2 h for the inducible maltose carrier and 0.8 h for the inducible α-methyl-d-glucoside carrier. The distinct nature of the two last-named carriers was thus supported. De-induction of the galactose carrier was enhanced in the presence of glucose plus cycloheximide but not of either substance alone. Chloramphenicol suppressed all effects of cycloheximide. In contrast to the enzymes of galactose metabolism, the induction of the glactose carrier was not under the control of a mitochondrial factor and took place in a ϱ−1 mutant. The system induced by maltose but not the one induced by α-methyl-d-glucoside was de-induced rapidly by the intervention of a cytoplasmsynthesized protein.Keywords
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