A predominant idiotype on anti-hen egg white lysozyme antibodies from diverse mouses strains.
- 1 September 1980
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 125 (3), 1017-1025
- https://doi.org/10.4049/jimmunol.125.3.1017
Abstract
A predominant idiotype was detected on almost all antibody molecules directed against the multideterminant antigen, hen egg white lysozyme (HEL). This idiotypic set was defined by rabbit and quinea pig antiidiotypic antisera raised against a rare cross-reactive antibody population isolated and from a heterogeneous BALB/c anti-human lysozyme (HUL) humoral response. This isiotype was fond on the majority of all HEL-reactive antibody from all strain tested; its presence was independent of Ig-1 allotype of H-2 haplotype. The idiotype was also present on the majority of anti-HEL antibody from nonresponder mice immunized with keyhole limpet hemocyanin coupled to HEL. The idiotype was not found on normal mouse immunoglobulin or on antibodies specific to two other proteins, HUL and ribonuclease, which are similar to HEl in size and charge. Individual idiotype-positive anti-HEL antibody populations exhibited specificity differences when analyzed for reactivity toward other avian lysozymes, suggesting that distinct clonal products shared the idiotype specificities. Fractionation of heterogeneous anti-HEL antibodies by preparative isoelectric focusing yielded distinct antibody fractions that were demonstrated to share the relevant idiotype determinants. The data are discussed in terms of the HEL peptide specificites of idiotype-positive and -negative antibodies.This publication has 12 references indexed in Scilit:
- Idiotypic cross-reactivity between antibodies of different specificities.The Journal of Experimental Medicine, 1978
- Preparation and characterization of anti‐framework antibodies to the heavy chain variable region (VH) of mouse immunoglobulinsEuropean Journal of Immunology, 1978
- Cross-Reactive Idiotypic Determinants on Murine Anti-(T,G)-A—L AntibodiesThe Journal of Immunology, 1978
- GENETIC-CONTROL OF IMMUNE-RESPONSE TO GAT TERPOLYMER .1. INTERSTRAIN AND INTERSPECIES CROSS-REACTIVE IDIOTYPE1978
- IDIOTYPIC ANALYSIS OF ANTI-GAT ANTIBODIES .1. PRESENCE OF COMMON IDIOTYPIC SPECIFICITIES IN BOTH RESPONDER AND NONRESPONDER MICE1978
- Absolute frequencies of lipopolysaccharide-reactive B cells producing A5A idiotype in unprimed, streptococcal A carbohydrate-primed, anti-A5A idiotype-sensitized and anti-A5A idiotype-suppressed A/J mice.The Journal of Experimental Medicine, 1977
- Structural aspects of immune recognition of lysozymes. III. T cell specificity restriction and its consequences for antibody specificityEuropean Journal of Immunology, 1976
- GENETIC-CONTROL OF IMMUNE-RESPONSE TO NUCLEASE .2. DETECTION OF IDIOTYPIC DETERMINANTS BY INHIBITION OF ANTIBODY-MEDIATED NUCLEASE INACTIVATION1976
- Isoelectric Focusing in Polyacrylamide Gel and its Application to ImmunoglobulinsNature, 1968
- CHROMATOGRAPHIC STUDIES ON LYSOZYMEJournal of Biological Chemistry, 1953