Cloning of a lincosamide resistance determinant from Streptomyces caelestis, the producer of celesticetin, and characterization of the resistance mechanism
- 1 August 1990
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 172 (8), 4710-4714
- https://doi.org/10.1128/jb.172.8.4710-4714.1990
Abstract
Self-resistance has been investigated in Streptomyces caelestis (producer of the lincosamide antibiotic celesticetin), from which a lincosamide resistance determinant (clr) has been isolated on a 1-kilobase DNA fragment and cloned in Streptomyces lividans. The clr product is a specific methylase which produces a single residue of N6-monomethyladenine in 23S rRNA at position 2058, thereby rendering the 50S ribosmal subunit resistant to the action of lincosamides.This publication has 17 references indexed in Scilit:
- Inducible Ribosomal RNA Methylation in Streptomyces lividans, Conferring Resistance to LincomycinMicrobiology, 1989
- Organization and nucleotide sequence analysis of a ribosomal RNA gene cluster from Streptomyces ambofaciensGene, 1989
- Use of a Fractionated, Coupled Transcription-Translation System in the Study of Ribosomal Resistance Mechanisms in Antibiotic-producing StreptomycesMicrobiology, 1989
- Ribosomal RNA methylation in Staphylococcus aureus and Escherichia coli: Effect of the “MLS” (erythromycin resistance) methylasePlasmid, 1985
- Methylation of 16S ribosomal RNA and resistance to aminoglycoside antibiotics in clones of Streptomyces lividans carrying DNA from Streptomyces tenjimariensisMolecular Genetics and Genomics, 1985
- Coupled transcription — translation in extracts of Streptomyces lividansMolecular Genetics and Genomics, 1984
- Translational Attenuation: The Regulation of Bacterial Resistance to the Macrolide-Lincosamide-Streptogramin B AntibioticCritical Reviews in Biochemistry, 1984
- Cloning and Expression of the Tyrosinase Gene from Streptomyces antibioticus in Streptomyces lividansMicrobiology, 1983
- Macrolide Resistance in Staphylococcus aureus : Inducers of Macrolide ResistanceAntimicrobial Agents and Chemotherapy, 1977
- Alteration of 23 S ribosomal RNA and erythromycin-induced resistance to lincomycin and spiramycin in Staphylococcus aureusJournal of Molecular Biology, 1973