Chemotherapy of Leukemia. V. Effects of 2,4,6-Triethylenimino-S-Triazine and Related Compounds on Transplanted Mouse Leukemia.

Abstract

42 compounds of which 39 could be classed as triazines and 3 as ethylenimine ring compounds were screened for chemotherapeutic activity against AK4 mouse leukemia. In a typical expt., 240 mice of Akm stock were injected intraperit. with 0.1 cc. of saline suspension containing 1 million cells. 48 hrs. after injn., the mice were divided into groups of 10 each, (2 sets of untreated controls, 2 sets of controls treated with a standard compound of known activity, 4-amino-N10-methyl-pteroyl-glutamic acid and 20 sets of mice treated with unknown compounds. Compounds were given intraperit. in maximum tolerated doses 3 times weekly for 10 doses. 2,4,6-triethylen-imino-S-triazine, 2,4-diethylenimino-6-amino-S-triazine, and hexamethylene diethylenurea significantly prolonged the survival time of mice injected with transplanted leukemia AK4. It appears that the chemotherapeutic activity of the above compounds can be attributed to the ethylenimine structure.