Fractionation of Polymeric Procyanidins from Lowbush Blueberry and Quantification of Procyanidins in Selected Foods with an Optimized Normal-Phase HPLC−MS Fluorescent Detection Method
- 17 July 2002
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 50 (17), 4852-4860
- https://doi.org/10.1021/jf020214v
Abstract
The polymeric procyanidins were fractionated from lowbush blueberry on a Sephadex LH-20 column. The degree of polymerization (DP) for the polymers was determined by thiolysis to be in a range of 19.9 to 114.1. Normal-phase HPLC analysis indicated that the polymeric procyanidins did not contain oligomeric procyanidins with DP < 10. The polymers eluted as a single peak at the end of the chromatogram. The normal-phase HPLC gradient was modified to improve the separation of procyanidin monomers through decamers and to elute all the polymers beyond those as a distinct peak. Monomers through decamers were quantified individually. All the polymers (DP > 10) were quantified using a mixture of purified polymers as an external standard. Polymers were found to be the dominant procyanidins in brown sorghum bran, cranberry, and blueberry. Thiolysis of the polymer peaks indicated that epicatechin was present as extension units in these foods, however, the composition of terminal units varied considerably between catechin and epicatechin, or an A-type dimer linkage in the case of cranberry. Keywords: Catechin; procyanidin; cocoa; blueberry; cranberry; sorghumKeywords
This publication has 16 references indexed in Scilit:
- High-performance liquid chromatography/mass spectrometry analysis of proanthocyanidins in food and beveragesMethods in Enzymology, 2001
- Proanthocyanidins and tannin-like compounds - nature, occurrence, dietary intake and effects on nutrition and healthJournal of the Science of Food and Agriculture, 2000
- Analysis of (+)-catechin, (−)-epicatechin and their 3′- and 4′-O-methylated analogsJournal of Chromatography B: Biomedical Sciences and Applications, 1999
- Identification of Procyanidins in Cocoa (Theobroma cacao) and Chocolate Using High-Performance Liquid Chromatography/Mass SpectrometryJournal of Agricultural and Food Chemistry, 1999
- Loss of Tannins and Other Phenolics from Willow Leaf LitterJournal of Chemical Ecology, 1998
- Method for Estimation of Proanthocyanidins Based on Their Acid Depolymerization in the Presence of NucleophilesJournal of Agricultural and Food Chemistry, 1997
- Oligomeric and polymeric procyanidins from grape seedsPhytochemistry, 1994
- Normal-phase high-performance liquid chromatographic separation of procyanidins from cacao beans and grape seedsJournal of Chromatography A, 1993
- Cacao procyanidins: major flavanoids and identification of some minor metabolitesPhytochemistry, 1991
- AUTOMATIC DETERMINATION OF OXYGENPublished by Elsevier ,1983