Histone mRNA in Xenopus laevis ovaries: identification of the H4 messenger
- 1 January 1977
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (4), 801-811
- https://doi.org/10.1093/nar/4.4.801
Abstract
RNA extracted from ovaries of adult Xenopus laevis and introduced into a wheat germ cell-free system, directs the synthesis of polypeptides co-electrophoresing with all five histones. By frationation of total ovary RNA through Sephadex G-200 and polyacrylamide gel, RNA was isolated that is translated into histones 4 (H4) only. In addition, this RNA labelled in vitro with [I125] hybridizes to a restriction fragment of cloned sea urchin DNA known to contain the H4 gene.Keywords
This publication has 20 references indexed in Scilit:
- Partial denaturation mapping of cloned histone DNA from the sea urchin Psammechinus miliarisNature, 1976
- Integration of eukaryotic genes for 5S RNA and histone proteins into a phage lambda receptorNucleic Acids Research, 1976
- Reiteration Frequency of the Histone Genes in the Genome of the Amphibian, Xenopus laevisEuropean Journal of Biochemistry, 1976
- A simple method for DNA restriction site mappingNucleic Acids Research, 1976
- Molecular analysis of the histone gene cluster of Psammechinus miliaris: I. Fractionation and identification of five individual histone mRNAsCell, 1976
- Histone messengers and histone genesCell, 1976
- Cytoplasmic origin of the so-called nuclear neutral histone proteaseBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- Histone synthesis in early amphibian development: Histone and DNA syntheses are not co-ordinatedJournal of Molecular Biology, 1974
- The use of RNA labeled in vitro with iodine-125 in molecular hybridization experimentsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
- An evaluation of film detection methods for weak β-emitters, particularly tritiumAnalytical Biochemistry, 1970