A Cytokinin-binding Protein from Wheat Germ
- 1 October 1979
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 64 (4), 594-599
- https://doi.org/10.1104/pp.64.4.594
Abstract
A cytokinin-binding protein has been isolated from wheat germ via ammonium sulfate precipitation, carboxymethyl Sephadex chromatography, and affinity chromatography on a column substituted with a derivative of kinetin riboside. On Sephadex G-200, the protein migrated with an apparent molecular weight of 122,000 daltons. The dissociation constant for kinetin was determined by equilibrium dialysis to be 1.2 micromolar; N6-benzylaminopurine and N6-(Δ2-isopentenyl)adenine were also strongly bound. Little affinity was exhibited toward either cis-zeatin or trans-zeatin.Keywords
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