Species Differences in Serum Glycoproteins of Man, Dog and Rabbit, by Paper Electrophoresis.

Abstract

A species-specific quantitative pattern of distribution of serum glycoproteins was established in normal human serum and in plasma from dogs and rabbits. After paper elctrophoresis the glycoprotein was stained with a modification of the periodic acid-Schiff method and evaluated with a densitometer. Normal human serum showed 3 main components: alpha-1 and beta 30% each, and alpha-2, 40%. In dogs, the total quantity of stainable carbohydrate was less. Alpha-2 glycoprotein accounted for 56% of the total stainable carbohydrate with alpha-1 and beta glycoprotein present in about equal amounts. In rabbits, a single alpha glycoprotein was the major component (86%). No alterations were seen in plasma of rabbits treated with various corticosteroids nor in hypercholesteremic rabbits. A gamma glycoprotein was noted in human serum in several disease states. Studies of serum glycoproteins could be correlated with simultaneous determination of proteins and lipoproteins.