Comparison in control and severely diabetic, alloxanized rats of phosphate fractions, glycogen and fat

Abstract

Homogeneous aliquots of powdered muscle were analyzed and levels of constituents calculated on a fat-free basis. When compared to muscle from control rats, muscle from severely diabetic, alloxanized rats showed a decrease in creatine phosphate, total acid soluble phosphate and total phosphate, with no change in inorganic phosphate, adenosine diphosphate plus adenosine triphosphate, or total acid soluble phosphate minus 7-minute hydrolyzable phosphate, creatine, noncollagenous protein or total nitrogen. There was an increase in the trichloroacetic acid extractable glycogen value in the muscle of the alloxan diabetic as compared to the control series. The concentration of fat in the muscle itself was higher in the diabetic than in the control series, although the total muscle fat had decreased in the diabetic series due to the decrease in percentage of body weight represented by muscle.