Analysis of sites of foot and mouth disease virus persistence in carrier cattle via the polymerase chain reaction
- 1 September 1994
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 136 (3-4), 299-307
- https://doi.org/10.1007/bf01321059
Abstract
This study was undertaken in order to explore possible sites of foot-and-mouth disease virus (FMDV) persistence during the carrier state. Tissue samples taken from experimentally infected animals at different times post-infection (p.i) were examined by conventional viral isolation and the polymerase chain reaction (PCR) technique. The analysis of samples from several organs taken from 17 bovines between 3 and 270 days p.i. allowed the following conclusions: 1) Virus present in oesophageal-pharyngeal fluids (OPF) during the carrier state originates in the pharynx as shown by the detection of antisense FMDV RNA by PCR, 2) PCR is more sensitive than standard virus isolation techniques and may be used for the rapid detection of FMDV in specimens obtained during the acute stage of FMD and for identification of persistently infected cattle.Keywords
This publication has 20 references indexed in Scilit:
- Evaluation of techniques to demonstrate foot-and-mouth disease virus in bovine tongue epithelium: Comparison of the sensitivity of cattle, mice, primary cell cultures, cryopreserved cell cultures and established cell linesVeterinary Microbiology, 1989
- Detection of Foot-and-Mouth disease virus with DNA probes in bovine esophageal-pharingeal fluidsArchiv für die gesamte Virusforschung, 1988
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Sequence of the viral replicase gene from foot-and-mouth disease virus C1-Santa Pau (C-S8)Gene, 1985
- Pathogenesis of foot-and-mouth disease: the lung as an additional portal of entry of the virusEpidemiology and Infection, 1976
- Some properties of carrier strains of foot-and-mouth disease virusArchiv für die gesamte Virusforschung, 1970
- Foot-And-Mouth DiseaseAnnual Review of Microbiology, 1968
- Growth of Foot-and-Mouth Disease Virus in Monolayer Cultures of Calf Thyroid CellsNature, 1966
- Studies on the carrier state of cattle exposed to foot-and-mouth disease virusEpidemiology and Infection, 1966
- Foot-and mouth diseases carriersVeterinary Record, 1965