The effect of iron status on glyceraldehyde 3-phosphate dehydrogenase expression in rat liver

Abstract
The influence of iron status on glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene transcription, mRNA levels and distribution was determined in whole liver samples from adult Wistar rats. While iron loading did not alter GAPDH expression, iron deficiency evoked a 2.3-fold increase in the steady-state level of liver GAPDH mRNA, but did not affect gene transcription or intracellular localisation of the message. Therefore, the over-expression of GAPDH mRNA in iron deficiency is probably due to increased message stability.