Stripping Film Technics for Histological Autoradiographs

Abstract

A new Eastman Kodak stripping film with a fast, fine grain emulsion, designated as NTB, is descr. for a and B autographs. A 10-u. emulsion is supported on a 10-/u. cellulose ester base. This combination is stripped from its thick cellulose support at the time of use. The tissue is placed either on the emulsion to eliminate backscatter, or on the cellulose ester base to eliminate chemical fogging, to prevent damage to the tissue during developing and fixing the emulsion, and to prevent staining the emulsion. Autographs of blood smears on glass slides are made by laying the film, base down, over the smear. The base separates the tissue from the emulsion. This permits observation and counting of all grains beneath heavily stained histological bodies but reduces resolution as compared with the technic of placing the tissue directly on the emulsion.