Utilization of rat lymphocytes for the in vitro chromosomal aberration assay
- 1 March 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Mutagenesis
- Vol. 4 (2), 147-153
- https://doi.org/10.1093/mutage/4.2.147
Abstract
In vitro cytogenetic assays are widely conducted to assess the mutagenic potential of chemicals. Chinese hamster ovary (CHO) cells or human lymphocytes are often used for these assays; however, these cell types have certain limitations. In order to evaluate an alternate cell system, cultured rat lymphocytes were treated for 4 h at 48 h of incubation with a variety of direct-and indirect-acting clastogens in the presence or absence of an exogenous mammalian activation system. Cytogenetic effects of in vitro physiological alterations such as medium hypertonicity or pH changes were also evaluated. The background aberration rate of rat lymphocytes is ∼ 2%, and they respond positively to both direct- and indirect-acting clastogens. In contrast to CHO cells, however, neither the hyperosmolality nor pH changes in the treatment media have significant effect on background aberrations. Unlike samples of human blood, rat blood can be collected under well-controlled environmental conditions. Because of the easy access to rat blood samples, the simplicity of culture, the reproducible nature of its in vitro growth, the positive response to known clastogens and negative response to media pH changes or hyperosmolalities, the rat lymphocyte in vitro chromosomal assay presented is an optimal system to assess the mutagenic potential of chemicals.This publication has 6 references indexed in Scilit:
- Studies on the ability of hypotonic solutions to induce chromosomal aberrations in V 79 cellTeratogenesis, Carcinogenesis, and Mutagenesis, 1987
- Clastogenicity in vitro of the Na, K, Ca and Mg salts of saccharin; and of magnesium chloride; consideration of significanceMutation Research, 1986
- Cytogenetic variability of lymphocytes from phenotypically normal men: Influence of age, smoking, season, and sample storageJournal of Toxicology and Environmental Health, 1985
- The incidence of spontaneous cytogenetic aberrations in lymphocytes cultured from normal humans for 48 and 72 hCanadian Journal of Genetics and Cytology, 1984
- Primary mutagenicity screening of food additives currently used in JapanFood and Chemical Toxicology, 1984
- Cytotoxicity and mutagenicity of dimethylnitrosamine in mammalian cells (CHO/HGPRT system): Enhancement by calcium phosphateEnvironmental Mutagenesis, 1982