PROMOTION BY DIETARY PHENOBARBITAL OF HEPATOCARCINOGENESIS BY 2-METHYL-N,N-DIMETHYL-4-AMINOAZOBENZENE IN RAT

Abstract

The hepatocarcinogenicity of 2-methyl-N,N-dimethyl-4-aminoazobenzene, previously shown to be noncarcinogenic in adult rats in the absence of further treatment, was observed by following a 1-6 wk period of feeding this dye to weanling rats with the dietary adminstration of 0.05% phenobarbital for up to 70 wk. Many large hepatocellular carcinomas developed in the phenobarbital-treated animals by 72 wk, whereas a very small number of tny neoplastic nodules, including 1 carcinoma, were seen in the rats not given this drug. The use of promoting agents following the short-term administration of weak carcinogens for the liver can be useful in demonstrating the initiating activity of such compounds. This system may be useful in the identification of such agents in the environment.