Calmodulin activates prokaryotic adenylate cyclase.
- 1 July 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (7), 3841-3844
- https://doi.org/10.1073/pnas.77.7.3841
Abstract
The adenylate cyclase of Bordetella pertussis is stimulated 100- to 1000-fold in a dose-dependent manner by calf brain calmodulin. The system has the following properties. The activation is prevented by ethylene glycol bis(.beta.-aminoethyl ether)-N,N,N'',N''-tetraacetic acid and restored by Ca2+. Oxidation of the methionine residues of calmodulin abolishes the ability to activate the cyclase. Trifluoperazine inhibits calmodulin-activated cyclase. A troponin C preparation stimulates the B. pertussis cyclase with < 0.01 the potency of calmodulin. Although calmodulin was not demonstrated in prokaryotes, this is an example of a (eukaryotic) calmodulin effect in a prokaryote.This publication has 26 references indexed in Scilit:
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