CHEMICAL ASSAY FOR “CORTIN”
- 1 February 1948
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 8 (2), 166-174
- https://doi.org/10.1210/jcem-8-2-166
Abstract
-A method was described which can be used satisfactorily for the quantitative estimation of urinary corti-costeroids. The procedure is sufficiently simple to be useful in clinical as well as in research studies. The extraction is carried out on acidified urine with chloroform. A partition between benzene and water is used and the aqueous fraction thus formed is oxidized with periodic acid. The formaldehyde formed by this reaction is distilled from interfering chromogens and the amt. of color formed with chromotropic acid is measured. The results obtained correspond in order of magnitude to other chemical methods applied to acidified urine.Keywords
This publication has 4 references indexed in Scilit:
- THE NEUTRAL LIPIDE-SOLUBLE REDUCING SUBSTANCES OF URINE AS AN INDEX OF ADRENAL CORTICAL FUNCTIONJournal of Biological Chemistry, 1946
- BIOLOGICAL ASSAY OF ADRENAL CORTICOIDS1Endocrinology, 1946
- STEROIDS .8. A COLORIMETRIC METHOD FOR THE ESTIMATION OF REDUCING STEROIDS1946
- THE COLORIMETRIC ASSAY OF URINARY CORTICOSTEROID-LIKE SUBSTANCESJournal of Biological Chemistry, 1945