Horizontal two‐dimensional electrophoresis of cerebrospinal fluid proteins with immobilized pH gradients in the first dimension
- 1 January 1990
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 11 (1), 91-92
- https://doi.org/10.1002/elps.1150110118
Abstract
A horizontal two‐dimensional electrophoresis method with immobilized pH gradient isoelectric focusing supplemented with carrier ampholytes in the first dimension was applied to cerebrospinal fluid (CSF) proteins. About 300 protein spots could be detected on the silver‐stained two‐dimensional maps of CSF samples. This highresolution method is a tool worthy of consideration for the research of CSF proteins and disease‐specific changes in different neurological disorders.This publication has 18 references indexed in Scilit:
- Analysis of porcine erythrocyte lysate proteins by horizontal two‐dimensional electrophoresis with immobilized pH gradient in the first dimensionElectrophoresis, 1989
- Two‐dimensional electrophoresis. Analysis of tubulin proteins and peptides in neuronal and non‐neuronal tissues using immobilized pH gradientsElectrophoresis, 1988
- Two‐dimensional electrophoresis. The current state of two‐dimensional electrophoresis with immobilized pH gradientsElectrophoresis, 1988
- Two‐dimensional electrophoresis. Dual‐label autoradiographic analysis of human skin fibroblast and myoblast proteins by two‐dimensional polyacrylamide gel electrophoresis using immobilised pH gradients in the first dimensionElectrophoresis, 1987
- Horizontal two‐dimensional electrophoresis with immobilized pH gradients in the first dimension in the presence of nonionic detergentElectrophoresis, 1987
- High resolution two‐dimensional polyacrylamide gel electrophoresis of basic myeloid polypeptides: Use of immobilized pH gradients in the first dimensionElectrophoresis, 1986
- Two‐dimensional analysis of membrane proteins with isoelectric focusing in immobilized pH gradients in the first dimensionElectrophoresis, 1986
- High‐resolution two‐dimensional electrophoresis of myofibrillar proteins with immobilized pH gradientsElectrophoresis, 1985
- Urine analysis by two‐dimensional gel eletrophoresis with isoelectric focusing in immobilized pH gradients in the first dimensionElectrophoresis, 1985
- Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver stainingElectrophoresis, 1985