Mass Transfer and Cholesterol Oxidase Kinetics in a Liquid-Liquid Two-Phase System
- 1 January 1989
- journal article
- research article
- Published by Taylor & Francis in Biocatalysis
- Vol. 2 (2), 97-120
- https://doi.org/10.3109/10242428909003651
Abstract
The kinetics of cholesterol oxidase and the effect of mass transfer in an aqueous-organic dispersion have been investigated. Cholesterol oxidase exhibits Michaelis-Menten behaviour in terms of substrate and enzyme concentrations. However, the observed rate of reaction depends on the nature and concentration of surfactants added, agitation rate, and organic phase composition. Analysis of the experimental data in terms of simultaneous mass transfer with reaction indicates that the system may operate in the slow reaction-diffusional regime.Keywords
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