EXTRACTION OF THYROTROPHIN FROM PITUITARY GLANDS, MOUSE PITUITARY TUMORS, AND BLOOD PLASMA BY PERCOLATION
- 1 July 1959
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 65 (1), 7-17
- https://doi.org/10.1210/endo-65-1-7
Abstract
A percolation method of extraction of thyrotrophin from dry powders of anterior pituitary glands, mouse pituitary tumors and blood plasma has been developed. The powder mixed with an equal weight of filter aid suspended in 95% ethanol was placed on a Buchner funnel and increasingly more aqueous solutions containing salt were run in succession through the filter cake in a stepwise manner. The TSH is removed with 57 or 67% ethanol solutions saturated with sodium chloride. The yields were estimated to be over 50% of the potency in about 2% of the weight, which is a concentration of the activity of about 30 times, whether from dried plasma or pituitary powder.Keywords
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