The size of collagen fibrils that stimulate platelet aggregation in human plasma
- 15 January 1980
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 186 (1), 5-12
- https://doi.org/10.1042/bj1860005
Abstract
Suspensions of collagen fibrils of different size were prepared from solutions of radioactive tropocollagen type I by either differential centrifugation or differential incubation at elevated temperature. The fractions were compared regarding their ability to stimulate human blood platelet aggregation in plasma, their binding to human platelets, and their morphology, as seen in EM. Although small particles with a sedimentation coefficient as low as 4.5 S bound to platelets, aggregation was not observed in the presence of collagen multimers and 6 protofibrils without visible cross-bands in stained specimens. The onset of platelet-aggregating activity before the appearance of turbidity in collagen solutions incubated at elevated temperature is due to the formation of a few banded fibrils; this early onset and the fibrils do not appear in collagen solutions that were ultracentrifuged before incubation.This publication has 19 references indexed in Scilit:
- Collagen-induced platelet aggregation and release. II critical size and structural requirements of collagenBiochimica et Biophysica Acta (BBA) - General Subjects, 1978
- Collagen-induced platelet aggregation: Native collagen quaternary structure is not an essential structural requirementThrombosis Research, 1978
- Identification of collagen α1(I) trimer in embryonic chick tendons and calvariaBiochemical and Biophysical Research Communications, 1977
- Platelet-aggregating activity of type I and type III collagens from human aorta and chicken skinBiochemical Journal, 1976
- Evidence for a Structural Requirement for the Aggregation of Platelets by CollagenJCI Insight, 1974
- Structural Characterization of a Soluble Fraction from Lens‐Capsule Basement MembraneEuropean Journal of Biochemistry, 1973
- Further evidence for a transport form of collagen. Its extrusion and extracellular conversion to tropocollagen in embryonic tendonFEBS Letters, 1971
- The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytesArchives of Biochemistry and Biophysics, 1963
- The formation of fibrils from collagen solutions 1. The effect of experimental conditions: kinetic and electron-microscope studiesBiochemical Journal, 1960
- A Citrate-Phosphate-Dextrose Solution for the Preservation of Human BloodAmerican Journal of Clinical Pathology, 1957