Purification of Leucine tRNA Isoaccepting Species from Soybean Cotyledons

Abstract
Of the 6 leucine isoaccepting tRNA species from soybean (Glycine max) cotyledons, 2 recognize U-beginning codons and contain cytokinin moieties in their structure. These same 2 isoaccepting species were shown to undergo quantitative changes in their relative amounts upon treatment with N6-benzyladenine in vivo. A procedure was developed for purification of the isoaccepting species of leucine tRNA from soybean cotyledons resulting in isoacceptors of minimum purity, calculated by amino acid acceptance capacity, of from 46-78% leucine tRNA.