Subcellular Localization of Chitinase and of Its Potential Substrate in Tomato Root Tissues Infected by Fusarium oxysporum f. sp. radicis-lycopersici
- 1 April 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 92 (4), 1108-1120
- https://doi.org/10.1104/pp.92.4.1108
Abstract
Antiserum raised against a tomato (Lycopersicon esculentum Mill.) chitinase (molecular mass of 26 kilodaltons) was used as a probe to study the subcellular localization of this enzyme in tomato root tissues infected with Fusarium oxysporum f. sp. radicis-lycopersici. A time-course experiment revealed that chitinase accumulated earlier in the incompatible interaction than in the compatible one. However, in both systems, chitinase deposition was largely correlated with pathogen distribution. The enzyme was found to accumulate in areas where host walls were in close contact with fungal cells. In contrast, the enzyme could not be detected in vacuoles and intracellular spaces. The substantial amount of chitinase found at the fungus cell surface supports the view of an antifungal activity. However, the preferential association of the enzyme with altered fungal wall areas indicates that chitinase activity is either preceded by the hydrolytic action of other enzymes such as .beta.-1,3-glucanases or coincides with these enzymes. The possibility that fungal glucans released through the action of .beta.-1,3-glucanases may act as elicitors of chitinase production is discussed.This publication has 27 references indexed in Scilit:
- Identification of Several Pathogenesis-Related Proteins in Tomato Leaves Inoculated with Cladosporium fulvum (syn. Fulvia fulva) as 1,3-β-Glucanases and ChitinasesPlant Physiology, 1989
- Antifungal Hydrolases in Pea TissuePlant Physiology, 1988
- Several “pathogenesis-related” proteins in potato are 1,3-β-glucanases and chitinasesProceedings of the National Academy of Sciences, 1988
- Ultrastructural localization of N-acetylglucosamine residues in the cell wall of Gigaspora margarita throughout its life-cycleBiology of the Cell, 1988
- Oligosaccharide Signalling in PlantsAnnual Review of Cell Biology, 1987
- Vacuolar Localization of Ethylene-Induced Chitinase in Bean LeavesPlant Physiology, 1984
- β-1,3-Endoglucanase from Soybean Releases Elicitor-Active Carbohydrates from Fungus Cell WallsPlant Physiology, 1983
- Proteinase inhibitor-inducing factor activity in tomato leaves resides in oligosaccharides enzymically released from cell wallsProceedings of the National Academy of Sciences, 1981
- Biosynthesis of cell walls of fungi.1979
- Preparation and Purification of Glucanase and Chitinase from Bean LeavesPlant Physiology, 1971